Biotechnology
Biotechnology
What's New
Documents updated in the last 60 days:
An application note illustrating the process for testing a particular cell type with the IRDye 800CW EGF for eventual use in vivo.
DocCategory: Odyssey,Cell-based_assays,In-cell_Western,
Subcategory(s): Application_Notes,Protocols,Technical_Information,
Date: 10/2008
Author: LI-COR
Datasheet featuring the anesthesia system and other accessories for the Pearl Imager.
DocCategory: Sales_Tools,Accessories,Pearl_Imager,Live_Animal_Imaging,
Subcategory(s): Data_Sheet
Date: 10/2008
Author: LI-COR
Schematic demonstrating how to connect the EZ Anesthesia System to the Pearl Imager.
DocCategory: Accessories,Odyssey,Pearl_Imager,Live_Animal_Imaging,
Subcategory(s): Manuals
Date: 10/2008
Author: LI-COR
Instruction manual for LI-COR Blot Washer
DocCategory: Aerius,Odyssey,Western_Blots,
Subcategory(s): Manuals
Date: 10/2008
Author: LI-COR Biosciences
Two-Color Protein Marker Pack Insert
DocCategory: Western_Blots
Subcategory(s): Pack_Inserts
Date: 10/2008
Author: LI-COR
Sample course guide for undergraduate training in DNA sequencing.
DocCategory: GEMF,Undergraduate,
Subcategory(s): GEMF_Application_Notes
Date: 9/2008
Author: Peter Blair
This technical note provides a variety of examples and analysis guidelines for analyzing in vivo data with Pearl Cam Software.
DocCategory: Pearl_Cam_Software
Subcategory(s): Technical_Information
Date: 9/2008
Author: LI-COR
IRDye 800CW Goat Anti-Rat Secondary Antibody
DocCategory: IRDyes,Odyssey,
Subcategory(s): Pack_Inserts
Date: 9/2008
Author: LI-COR
IRDye Infrared Dye brochure.
DocCategory: IRDye_Reagents,Proteomics_Reagents,Cancer,Microscopy,IRDyes,
Subcategory(s): Brochures
Date: 9/2008
Author: LI-COR
Pack insert for IRDye 800CW Donkey Anti-Chicken Secondary Antibody
DocCategory: IRDyes,Odyssey,
Subcategory(s): Pack_Inserts
Date: 9/2008
Author: LI-COR
Approximate cost of Sequencing
DocCategory: 4300,GEMF,Undergraduate,
Subcategory(s): Cost_Analyses
Date: 9/2008
Author: Jackie Potts
Approximate cost for Microsatellite Analysis.
DocCategory: 4300,GEMF,Undergraduate,
Subcategory(s): Cost_Analyses
Date: 9/2008
Author: Jackie Potts
Non-lot specific pack insert information.
DocCategory: IRDyes,Odyssey,
Subcategory(s): Pack_Inserts
Date: 9/2008
Author: LI-COR Biosciences
Instructions on the installation of the Pearl Imager
DocCategory: Pearl_Imager
Subcategory(s): Manuals,Technical_Information,
Date: 9/2008
Author: LI-COR
Instructions on the replacement of the desiccant on the Pearl Imager.
DocCategory: Pearl_Imager
Subcategory(s): Technical_Information
Date: 9/2008
Author: LI-COR
Instructions on removing a mouse from the Pearl Imager in the event of an escape.
DocCategory: Pearl_Imager,Live_Animal_Imaging,
Subcategory(s): Technical_Information
Date: 9/2008
Author: LI-COR
Webinars focused on the imaging of small animals.
DocCategory: IRDyes,Odyssey,Pearl_Imager,Live_Animal_Imaging,
Subcategory(s): Webinars
Date: 9/2008
Author: LI-COR
Reprint of a review article on the development of contrast agents for small animal imaging.
DocCategory: IRDyes,Odyssey,Pearl_Imager,Live_Animal_Imaging,
Subcategory(s): Publications
Date: 9/2008
Author: LI-COR
Pearl Embedded Software Update. Some portions of this software fall under Open Source Licences. See the Pearl Cam Manual for more information. For source code downloads, browse the Pearl ftp directory at ftp://ftp.licor.com/perm/bio/pearl
DocCategory: Pearl_Cam_Software,Pearl_Imager,
Subcategory(s): Software_Updates
Date: 9/2008
Author: LI-COR
IRDye® 800CW 2-DG is a fluorescent optical imaging agent that has been shown to be reactive with implanted tumors derived from a number of cell lines..
DocCategory: Live_Animal_Imaging
Subcategory(s): Brochures
Date: 9/2008
Author: LI-COR
Approximate cost for AFLP Analysis
DocCategory: 4300,GEMF,Undergraduate,
Subcategory(s): Cost_Analyses
Date: 9/2008
Author: Jackie Potts
IRDye 680 Goat Anti-Rat IgG, 0.5mg
DocCategory: Proteomics_Reagents
Subcategory(s): Pack_Inserts
Date: 9/2008
Author: LI-COR
IRDye 800CW Goat Anti-Rat IgG, 0.5 mg
DocCategory: Proteomics_Reagents
Subcategory(s): Pack_Inserts
Date: 9/2008
Author: LI-COR
Pack Insert for IRDye 680 Goat Anti-Rabbit secondary antibody (926-32221)
DocCategory: Aerius,IRDye_Reagents,Odyssey,Proteomics_Reagents,IRDyes,
Subcategory(s): Pack_Inserts
Date: 9/2008
Author: LI-COR Biosciences
IRDye 680 Goat Anti-Rat Secondary Antibody
DocCategory: IRDyes,Odyssey,
Subcategory(s): Pack_Inserts
Date: 9/2008
Author: LI-COR
IRDye 800CW Donkey Anti-Chicken IgG, 0.5 mg
DocCategory: Proteomics_Reagents
Subcategory(s): Pack_Inserts
Date: 9/2008
Author: LI-COR
Instructions for recovering a mouse that has escaped the Pearl Imager imaging bed
DocCategory: Pearl_Imager
Subcategory(s): Manuals
Date: 9/2008
Author: LI-COR Biosciences
Non-lot specific pack insert information.
DocCategory: IRDyes
Subcategory(s): Pack_Inserts
Date: 9/2008
Author: LI-COR Biosciences
Non-lot specific pack insert information.
DocCategory: IRDyes
Subcategory(s): Pack_Inserts
Date: 9/2008
Author: LI-COR Biosciences
Pack insert for IRDye 800CW Donkey Anti-Rabbit secondary antibody (P/N 926-32213)
DocCategory: Aerius,IRDye_Reagents,Odyssey,Proteomics_Reagents,IRDyes,
Subcategory(s): Pack_Inserts
Date: 9/2008
Author: LI-COR Biosciences
Pack Insert for IRDye 800CW Donkey Anti-Mouse secondary antibody (P/N 926-32212)
DocCategory: Aerius,IRDye_Reagents,Odyssey,Proteomics_Reagents,IRDyes,
Subcategory(s): Pack_Inserts
Date: 9/2008
Author: LI-COR Biosciences
Approximate cost for gel chemistry
DocCategory: 4300,GEMF,Undergraduate,
Subcategory(s): Cost_Analyses
Date: 9/2008
Author: Jackie Potts
Pack Insert for IRDye 800CW donkey anti-goat secondary antibody (926-32214)
DocCategory: Aerius,IRDyes,Odyssey,
Subcategory(s): Pack_Inserts
Date: 9/2008
Author: LI-COR Biosciences
Pack insert for IRDye 680 Donkey Anti-Mouse secondary antibody (926-32222)
DocCategory: Aerius,IRDye_Reagents,Odyssey,Proteomics_Reagents,IRDyes,
Subcategory(s): Pack_Inserts
Date: 9/2008
Author: LI-COR Biosciences
Detailed protocol for Western Blot detection in the Odyssey Imaging System. Updated July 2007 with Newblot Stipping Buffers
DocCategory: Odyssey,Western_Blots,
Subcategory(s): Protocols
Date: 9/2008
Author: LI-COR
PDF instructions for installing Odyssey 2.1 application software. WARNING: Version 2.1 uses file formats not compatible with earlier versions.
DocCategory: Odyssey
Subcategory(s): Manuals,Software_Updates,
Date: 9/2008
Author: LI-COR Biosciences
Mikel Stevens of Brigham Young University presents ideas for undergraduate research programs
DocCategory: 4300,GEMF,Undergraduate,
Subcategory(s): GEMF_Application_Notes
Date: 9/2008
Author: Mikel Stevens
3rd place winner in 2008 Student poster competition. This is a PPT file.
DocCategory: 4300,GEMF,Undergraduate,
Subcategory(s): Presentations_and_Posters
Date: 9/2008
Author: Tapadia, Sroya, Khan, Khlebnikova
Pack Insert for IRDye 800CW Goat Anti-Rabbit secondary antibody (926-32211)
DocCategory: Aerius,IRDye_Reagents,Odyssey,Proteomics_Reagents,Western_Blots,IRDyes,
Subcategory(s): Pack_Inserts
Date: 9/2008
Author: LI-COR Biosciences
Pack Insert for IRDye 800CW Goat Anti-Mouse secondary antibody (926-32210)
DocCategory: Aerius,IRDye_Reagents,Odyssey,Proteomics_Reagents,Western_Blotting,IRDyes,
Subcategory(s): Pack_Inserts
Date: 9/2008
Author: LI-COR Biosciences
Detailed protocol for adapting Panomics TransSignal Arrays for detection using the Odyssey System
DocCategory: Odyssey,Protein_Arrays,
Subcategory(s): Protocols
Date: 9/2008
Author: LI-COR Biosciences
Detailed protocol for adapting Active Motif TransAM assays for detection with the Odyssey System.
DocCategory: Odyssey,Transcription_Factor_Assays,
Subcategory(s): Protocols
Date: 9/2008
Author: LI-COR Biosciences
Detailed protocol for staining agarose gels with the nucleic acid-binding dye Syto60
DocCategory: Odyssey,Other_Applications,
Subcategory(s): Protocols
Date: 9/2008
Author: LI-COR Biosciences
Optical imaging is a rapidly developing field of research aimed at non-invasively interrogating animals for disease progression, determining the effects of a drug on a particular pathology, assessing the pharmacokinetic behavior of a drug, or identifying molecular biomarkers of disease. One of the key components of molecular imaging is the development of specific, targeted imaging contrast agents to assess these biological processes. The development of robust fluorochrome-labeled optical agents is a process that is often underestimated in terms of its complexity. Although many studies describe the use of these agents, guidelines for their development and testing are not readily available. This review outlines some of the general principles that are important when developing and using fluorochrome-labeled optical contrast agents for oncology investigations in animals.
DocCategory: IRDye_Reagents,Cancer,Live_Animal_Imaging,Small_Animal_Imaging,IRDyes,
Subcategory(s): Publications
Date: 9/2008
Author: Mike Olive
FAQs for Odyssey In-Cell Westerns and suspension cells.
DocCategory: Odyssey
Subcategory(s): Protocols
Date: 9/2008
Author: LI-COR Biosciences
Protocol for adapting Super Arrays/gene expression arrays on the Odyssey.
DocCategory: Odyssey,Oligonucleotide_arrays,
Subcategory(s): Protocols
Date: 9/2008
Author: LI-COR Biosciences
GEArray Q Series Gene Expression Arrays Protocol, superarray, super array
DocCategory: Odyssey
Subcategory(s): Protocols
Date: 9/2008
Author: LI-COR Biosciences
GEMF Winning Proposal
DocCategory: GEMF,Undergraduate,
Subcategory(s): Sample_Winning_Proposals
Date: 9/2008
Author: Jackie Potts
NewBlot Stripping Buffer for PVDF membranes
DocCategory: Aerius,Odyssey,Proteomics_Reagents,Western_Blots,Western_Blotting,
Subcategory(s): Pack_Inserts
Date: 9/2008
Author: LI-COR Biosciences
Pack insert for Newblot Stripping Buffer for PVDF Membranes (P/N 928-40032)
DocCategory: Aerius,Odyssey,Proteomics_Reagents,
Subcategory(s): Pack_Inserts
Date: 9/2008
Author: LI-COR Biosciences
Pack Insert for NewBlot Stripping Buffer for Nitrocellulose Membranes (P/N 928-40030)
DocCategory: Aerius,Odyssey,Proteomics_Reagents,Western_Blots,Western_Blotting,
Subcategory(s): Pack_Inserts
Date: 9/2008
Author: LI-COR Biosciences
Tips for detecting the Panorama Mouse/Rat Tissue Extract Protein Array on the Odyssey System.
DocCategory: Odyssey,Protein_Arrays,
Subcategory(s): Protocols
Date: 9/2008
Author: LI-COR Biosciences
LI-COR Changes Service Support Structure for Older DNA Systems
DocCategory: 4000
Date: 9/2008
Author: LI-COR Biosciences
Tech Note providing tips and suggestions for scanning a mouse on the Odyssey System
DocCategory: Odyssey
Subcategory(s): Protocols
Date: 9/2008
Author: LI-COR Biosciences
Using Sapphire700 (#928-40022) and DRAQ5 for Cell Number Normalization
DocCategory: Aerius,Odyssey,Proteomics_Reagents,In-cell_Western,In-Cell_Western,
Subcategory(s): Pack_Inserts
Date: 9/2008
Author: LI-COR Biosciences
GEMF Winning Proposal
DocCategory: 4300,GEMF,Undergraduate,
Subcategory(s): Sample_Winning_Proposals
Date: 9/2008
Author: Jackie Potts
Specifications for Saga MX 3.3.
DocCategory: 4000,4200,4300,Saga,AFLP,
Subcategory(s): Brochures
Date: 9/2008
Author: LI-COR
Specifications and information about Saga GT 3.3
DocCategory: 4000,4200,4300,Saga,Microsatellites,
Subcategory(s): Technical_Information,Brochures,
Date: 9/2008
Author: LI-COR
2008 1st place winner in student poster competition. This is PPT file.
DocCategory: 4300,GEMF,Undergraduate,
Subcategory(s): Presentations_and_Posters
Date: 9/2008
Author: Relebohile Sekonyela
Detailed protocol for adapting RayBio Cytokine Antibody Arrays for detection using the Odyssey System
DocCategory: Odyssey,Protein_Arrays,
Subcategory(s): Protocols
Date: 9/2008
Author: LI-COR
Datasheet for the IRDye QC-1 NHS Ester.
DocCategory: IRDye_Reagents,Proteomics_Reagents,Protease_Assay,IRDyes,
Subcategory(s): Brochures,Data_Sheet,
Date: 9/2008
Author: LICOR
Pack insert for the IRDye QC-1 NHS Ester (Quencher dye).
DocCategory: IRDye_Reagents,Proteomics_Reagents,Live_Animal_Imaging,Small_Animal_Imaging,IRDyes,
Subcategory(s): Pack_Inserts
Date: 9/2008
Author: LI-COR
Publications containing Odyssey® Infrared Imaging System data
DocCategory: Odyssey,Sales_Tools,
Subcategory(s): Publications,Distributor,Distributor_Resource,
Date: 9/2008
Author: Jeffrey Pearson
Poster by Chen and Olive PN 979-06809
DocCategory: Odyssey
Subcategory(s): Presentations_and_Posters
Date: 9/2008
Author: LI-COR Biosciences
Alterations in glycosyl epitopes play key roles in tumor progression. Epidermal growth factor receptor (EGFR) is a well-known glycoprotein that is currently targeted for anticancer therapy.1 We used Con A, WGA and UEA-I to detect the presence of carbohydrates on EGFR in Western blot format and employed near-infrared (IR) fluorophores as the detection mechanism.
DocCategory: Odyssey
Subcategory(s): Presentations_and_Posters
Date: 9/2008
Author: Champoux, et. al.
Monitoring Progression of Prostate Tumors in Mice by Receptor-Targeted Near Infrared Optical Imaging
Poster by Kovar, Johnson, Volcheck, Chen, Simpson PN 979-08639
DocCategory: Odyssey
Subcategory(s): Presentations_and_Posters
Date: 9/2008
Author: LI-COR Biosciences
Poster presentation on Protease detection using LI-COR IRDye 800RS-labeled casein.
DocCategory: Aerius,Odyssey,Protease_Assay,
Subcategory(s): Presentations_and_Posters
Date: 9/2008
Author: LI-COR
Poster by Kovar, Johnson, Volcheck, Chen, Simpson PN 979-08638
DocCategory: Odyssey
Subcategory(s): Presentations_and_Posters
Date: 9/2008
Author: LI-COR Biosciences
Poster by Chen, Urh, Schutz, Olive PN 979-06774
DocCategory: Odyssey
Subcategory(s): Presentations_and_Posters
Date: 9/2008
Author: LI-COR Biosciences
Application Note for using the RepliGen Protein A ELISA Assay on the Odyssey System.
DocCategory: ELISA/FLISA
Subcategory(s): Application_Notes
Date: 9/2008
Author: LI-COR Biosciences
IRDye 680 Donkey Anti-Chicken Secondary Antibody
DocCategory: IRDyes,Odyssey,
Subcategory(s): Pack_Inserts
Date: 9/2008
Author: LI-COR
EMSA Oligo, HIF-1 (IRDye 700)
DocCategory: Odyssey,Proteomics_Reagents,Gel-shift_assays_/_EMSA,Transcription_Factor_Assays,Oligos,
Subcategory(s): Pack_Inserts
Date: 9/2008
Author: LI-COR Biosciences
Pack insert for the IRDye 800RS Casein Protease Substrate (926-08587).
DocCategory: Aerius,IRDye_Reagents,Odyssey,Proteomics_Reagents,Other_Applications,IRDyes,
Subcategory(s): Pack_Inserts
Date: 9/2008
Author: LI-COR
Pack insert for the trial size of IRDye 800RS Casein Protease Substrate (926-09116).
DocCategory: Aerius,IRDye_Reagents,Odyssey,Proteomics_Reagents,Other_Applications,IRDyes,
Subcategory(s): Pack_Inserts
Date: 9/2008
Author: LI-COR
Pack insert for the IRDye 800CW NHS Ester.
DocCategory: IRDye_Reagents,IRDyes,
Subcategory(s): Pack_Inserts,Protocols,
Date: 9/2008
Author: LI-COR
Pack insert for the IRDye 800CW Carboxylate (929-08972).
DocCategory: Aerius,IRDye_Reagents,Odyssey,Proteomics_Reagents,In-cell_Western,Small_Animal_Imaging,IRDyes,
Subcategory(s): Pack_Inserts
Date: 9/2008
Author: LI-COR
Pack insert for the IRDye 800CW BoneTag Optical Probe.
DocCategory: IRDye_Reagents,Pearl_Imager,Live_Animal_Imaging,Small_Animal_Imaging,IRDyes,
Subcategory(s): Pack_Inserts
Date: 9/2008
Author: LICOR
Pack insert for the IRDye 700DX Protein Labeling Kit - Microscale kit.
DocCategory: IRDye_Reagents,Proteomics_Reagents,IRDyes,
Subcategory(s): Pack_Inserts
Date: 9/2008
Author: LI-COR Biosciences
Pack insert for the IRDye 700DX Protein Labeling Kit - Low MW .
DocCategory: IRDye_Reagents,Proteomics_Reagents,IRDyes,
Subcategory(s): Pack_Inserts
Date: 9/2008
Author: LI-COR Biosciences
Pack insert for the IRDye 700DX Protein Labeling Kit - High MW kit.
DocCategory: IRDye_Reagents,Proteomics_Reagents,Live_Animal_Imaging,Small_Animal_Imaging,IRDyes,
Subcategory(s): Pack_Inserts
Date: 9/2008
Author: LI-COR Biosciences
IRDye 680 Protein Labeling Kit - Microscale Kit pack insert.
DocCategory: IRDye_Reagents,Proteomics_Reagents,IRDyes,
Subcategory(s): Pack_Inserts
Date: 9/2008
Author: LI-COR
Pack insert for IRDye 680 Protein Labeling Kit for proteins with high molecular weight (928-38052).
DocCategory: Aerius,IRDye_Reagents,Odyssey,Proteomics_Reagents,IRDyes,
Subcategory(s): Pack_Inserts
Date: 9/2008
Author: LI-COR
Pack insert for the IRDye 680 BoneTag Optical Probe.
DocCategory: IRDye_Reagents,MousePOD,Odyssey,Pearl_Imager,Live_Animal_Imaging,Small_Animal_Imaging,IRDyes,
Subcategory(s): Pack_Inserts
Date: 9/2008
Author: LI-COR
Pack insert for IRDye 800CW 2-DG Optical Probe
DocCategory: IRDye_Reagents,MousePOD,Odyssey,Pearl_Imager,Cancer,Small_Animal_Imaging,Animal/Molecular_Imaging_Training,
Subcategory(s): Pack_Inserts
Date: 9/2008
Author: LI-COR
Fold-out installation sheet for Pearl Imager and Pearl Cam Software
DocCategory: Pearl_Cam_Software,Pearl_Imager,
Subcategory(s): Manuals
Date: 9/2008
Author: LI-COR Biosciences
Operator's manual for Pearl Imager and Pearl Cam software
DocCategory: Pearl_Cam_Software,Pearl_Imager,
Subcategory(s): Manuals
Date: 9/2008
Author: LI-COR Biosciences
Replacement instructions for the desiccant in the Pearl CCD camera housing.
DocCategory: Pearl_Imager
Subcategory(s): Manuals,Pack_Inserts,
Date: 9/2008
Author: LI-COR Biosciences
Cytokine antibody arrays on the Odyssey System
DocCategory: Odyssey
Subcategory(s): Protocols
Date: 9/2008
Author: LI-COR Biosciences
Odyssey User Guide Version 3.0
DocCategory: Odyssey,Odyssey_Software,
Subcategory(s): Manuals
Date: 9/2008
Author: LI-COR Biosciences
Odyssey application software manual version 2.1
DocCategory: Odyssey,Odyssey_Software,
Subcategory(s): Manuals
Date: 9/2008
Author: LI-COR Biosciences
Odyssey Tutorial Manual version 2.1
DocCategory: Odyssey,Odyssey_Software,
Subcategory(s): Manuals
Date: 9/2008
Author: LI-COR Biosciences
Complete Odyssey protocols manual with all protocols.
DocCategory: Odyssey
Subcategory(s): Manuals,Protocols,
Date: 9/2008
Author: LI-COR Biosciences
Basic instrument operation and maintenence information for the Odyssey Infrared Imager, and access via Internet browser. Version 2.1.
DocCategory: Odyssey
Subcategory(s): Manuals
Date: 9/2008
Author: LI-COR Biosciences
Guide for in vivo imaging on the Odyssey System using the MousePOD accessory. Version 2.1
DocCategory: Odyssey
Subcategory(s): Manuals
Date: 9/2008
Author: LI-COR Biosciences
Odyssey hardware and computer installation in a stand-alone configuration or network environment. Version 2.1
DocCategory: Odyssey
Subcategory(s): Manuals
Date: 9/2008
Author: LI-COR
In-Cell Western Assay for Phospho-p38 Detection in HeLa Cells in Response to Anisomycin
DocCategory: Odyssey,Cell-based_assays,In-cell_Western,
Subcategory(s): Protocols
Date: 9/2008
Author: LI-COR Biosciences
Odyssey software tutorial manual for version 3.0 software
DocCategory: Odyssey,Odyssey_Software,
Subcategory(s): Manuals
Date: 9/2008
Author: LI-COR Biosciences
Odyssey Operator's manual revised for version 3.0 software
DocCategory: Odyssey
Subcategory(s): Manuals
Date: 9/2008
Author: LI-COR Biosciences
Odyssey In Vivo Imaging Guide revised for version 3.0 software
DocCategory: Odyssey,Odyssey_Software,
Subcategory(s): Manuals
Date: 9/2008
Author: LI-COR Biosciences
Northern blot protocol using biotinylated probes
DocCategory: Odyssey,Northern_Blots,
Subcategory(s): Protocols
Date: 9/2008
Author: LI-COR Biosciences
Datasheet for the HIV-1 and b-secretase protease assays
DocCategory: Aerius,IRDye_Reagents,Odyssey,Proteomics_Reagents,
Subcategory(s): Brochures
Date: 9/2008
Author: LI-COR
User Guide, describing operation and maintenance of the MPX.
DocCategory: MPX
Subcategory(s): Manuals
Date: 9/2008
Author: LI-COR
Poster presentation on the application of high-throughput Western blotting.
DocCategory: Odyssey,Western_Blots,Western_Blotting,IRDyes,
Subcategory(s): Presentations_and_Posters,MPX,
Date: 9/2008
Author: LI-COR
Fold-out installation sheet describing the installation of a MousePOD on an Odyssey instrument
DocCategory: Odyssey,Accessories,Odyssey,
Subcategory(s): Manuals
Date: 9/2008
Author: LI-COR Biosciences
Single page data sheet on MousePOD accessory for the Odyssey System. PN 982-08470
DocCategory: Odyssey
Subcategory(s): Presentations_and_Posters
Date: 9/2008
Author: LI-COR Biosciences
An article featuring a Northern blots protocol developed by LI-COR's Dr. Kristi Ambroz.
DocCategory: Odyssey,Northern_Blots,
Subcategory(s): Protocols,Publications,
Date: 9/2008
Author: Jane M. Liu and David R. Liu
Publications containing Odyssey® Infrared Imaging System data
DocCategory: Odyssey,Sales_Tools,
Subcategory(s): Publications,Distributor,Distributor_Resource,
Date: 9/2008
Author: Neeraj Asundi
Fluorescent dyes provide important labeling tools for biological applications. As automated DNA sequencing continues to evolve with massively parallel methods and single molecule detection, fluorescence continues to play an important role in many of these systems.
DocCategory: IRDye_Reagents,Proteomics_Reagents,4300,IRDyes,
Subcategory(s): Publications,Technical_Information,
Date: 9/2008
Author: Osterman & Schutz-Geschwender
Protocol for in-gel detection of protein targets without blotting
DocCategory: Odyssey,In-gel_Western,
Subcategory(s): Protocols
Date: 9/2008
Author: LI-COR Biosciences
Application note on diet considerations for mice when doing small animal imaging.
DocCategory: MousePOD,Odyssey,Pearl_Imager,Live_Animal_Imaging,Small_Animal_Imaging,
Subcategory(s): Application_Notes,Technical_Information,
Date: 9/2008
Author: LICOR
ICW protocol for the IC50 determination of the MEK inhibitor U0126 on NIH3T3 cells
DocCategory: Odyssey,Cell-based_assays,In-cell_Western,
Subcategory(s): Protocols
Date: 9/2008
Author: LI-COR Biosciences
Odyssey ICW sample protocol for PMA-induced ERK Activation in Suspension Cell Lines
DocCategory: Odyssey,Cell-based_assays,In-cell_Western,
Subcategory(s): Protocols
Date: 9/2008
Author: LI-COR Biosciences
Sample protocol with data samples of PMA-induced ERK activation in suspension cell lines.
DocCategory: Odyssey
Subcategory(s): Protocols
Date: 9/2008
Author: LI-COR Biosciences
ICW protocol for the quantitative determination of AKT phosphorylation in NIH3T3 cells in response to PDGF-BB stimulation.
DocCategory: Odyssey,Cell-based_assays,In-cell_Western,
Subcategory(s): Protocols
Date: 9/2008
Author: LI-COR Biosciences
ICW protocol for the IC50 determination of the tyrosine kinase inhibitor PD168393 on multiple targets simultaneously
DocCategory: Odyssey,Cell-based_assays,In-cell_Western,
Subcategory(s): Protocols
Date: 9/2008
Author: LI-COR Biosciences
ICW protocol for the quantitative determination of p53 phosphorylation in COS cells in response to stimulation with hydroxyurea
DocCategory: Odyssey,Cell-based_assays,In-cell_Western,
Subcategory(s): Protocols
Date: 9/2008
Author: LI-COR Biosciences
Protocol for In-Cell Western Assay Kit I or Kit II
DocCategory: Aerius,IRDye_Reagents,Odyssey,Proteomics_Reagents,In-cell_Western,
Subcategory(s): Protocols
Date: 9/2008
Author: LI-COR Biosciences
ICW protocol for the quantitative determination ERK phosphorylation in HeLa cells in response to EGF stimulation.
DocCategory: Odyssey,Cell-based_assays,In-cell_Western,
Subcategory(s): Protocols
Date: 9/2008
Author: LI-COR Biosciences
An Odyssey assay for testing a particular cell type with IRDye 800CW EGF Optical Probe for eventual use in vivo.
DocCategory: In-cell_Western
Subcategory(s): Protocols
Date: 9/2008
Author: LI-COR Biosciences
ICW protocol for the quantitative determination of apoptosis (cleaved caspase-3) in HeLa cells in response to Anisomycin treatment.
DocCategory: Odyssey,Cell-based_assays,In-cell_Western,
Subcategory(s): Protocols
Date: 9/2008
Author: LI-COR Biosciences
ICW protocol for the quantitative determination of ERK phosphorylation in NIH3T3 cells in response to aFGF-1 stimulation.
DocCategory: Odyssey,Cell-based_assays,In-cell_Western,
Subcategory(s): Protocols
Date: 9/2008
Author: LI-COR Biosciences
ICW protocol for the quantitative determination of EGF receptor and ERK phosphorylation in A431 cells.
DocCategory: Odyssey,In-cell_Western,
Subcategory(s): Protocols
Date: 9/2008
Author: LI-COR Biosciences
Accurate and reliable means of variety identification are necessary to assess purity of seed supplies, to support claims relating to plant breeders’ rights and, in Canada, to provide quality assurances in the grain handling system. A single, multiplexed set of seven simple-sequence-repeat (SSR) markers was found to uniquely identify all 18 durum wheat varieties that have been developed in Canada and are currently, or were formerly, registered for commercial production. Significant features of this multiplexed set include an allele that is specific, within Canadian durum varieties, to those having high gluten strength, and redundancy that was included in an effort to increase the capacity to accommodate future varieties. In combination with a reasonably rapid individual-kernel DNA extraction protocol and automated allele calling, this marker system offers a higher resolution alternative to complement established protein-based variety identification methods.
DocCategory: 4000,4200,4300,Microsatellites,Plant,
Subcategory(s): Publications,Wheat,
Date: 9/2008
Author: Daniel Perry
This paper reviews a newly developed miniature dual-axes confocal microscope. It has been used for subsurface imaging of biological tissues with 5–7 u m resolution. Reflectance and fluorescence images were obtained with a laser source at 785 nm and the IRDye 800CW.
DocCategory: IRDye_Reagents,Microscopy,IRDyes,
Subcategory(s): Publications
Date: 9/2008
Author: Jonathan Liu
Photon penetration into living tissue is highly dependent on the absorption and scattering properties of tissue components. The near-infrared region of the spectrum offers certain advantages for photon penetration, and both organic and inorganic fluorescence contrast agents are now available for chemical conjugation to targeting molecules. This review focuses on those parameters that affect image signal and background during in vivo imaging with near-infrared light and exogenous contrast agents. Recent examples of in vivo near-infrared fluorescence imaging of animals and humans are presented, including imaging of normal and diseased vasculature, tissue perfusion, protease activity, hydroxyapatite and cancer.
DocCategory: IRDye_Reagents,Proteomics_Reagents,Small_Animal_Imaging,IRDyes,
Subcategory(s): Publications
Date: 9/2008
Author: John V. Frangioni
In vertebrates, the development and integrity of the skeleton requires hydroxyapatite (HA) deposition by osteoblasts. HA deposition is also a marker of, or a participant in, processes as diverse as cancer and atherosclerosis. At present, sites of osteoblastic activity can only be imaged in vivo using -emitting radioisotopes. The scan times required are long, and the resultant radioscintigraphic images suffer from relatively low resolution. We have synthesized a near-infrared (NIR) fluorescent bisphosphonate derivative that exhibits rapid and specific binding to HA in vitro and in vivo. We demonstrate NIR light?based detection of osteoblastic activity in the living animal, and discuss how this technology can be used to study skeletal development, osteoblastic metastasis, coronary atherosclerosis, and other human diseases.
DocCategory: IRDye_Reagents,Proteomics_Reagents,Cancer,Small_Animal_Imaging,IRDyes,
Subcategory(s):