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Odyssey:  Protocols

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Found 41 documents.

Biotin Streptavidin Northern Blotting Protocol v2.0 - Kristi Ambroz (7/2010)
Original protocol detailing the Odyssey Biotin Streptavidin Northern method.

Cayman Chemical PPAR Transcription Factor Kit Assay on the Odyssey System v9402 - LI-COR Biosciences (9/2008)
Cayman PPAR Transcription Factor Assays on the Odyssey System

Discovery of a mRNA mitochondrial localization element in Saccharomyces cerevisiae by nonhomologous random recombination and in vivo selection v1.0 - Jane M. Liu and David R. Liu (9/2008)
An article featuring a Northern blots protocol developed by LI-COR's Dr. Kristi Ambroz.

Electrophoretic Mobility Shift Assay (EMSA) Using IRDye™ Oligonucleotides v7487 - LI-COR Biosciences (9/2008)
EMSA protocol for IRDye-labeled oligonucleotides

FLISA (ELISA) for Odyssey v8353.0 - LI-COR Biosciences (9/2008)
Guidelines and recommendations for fluorescence-based detection of ELISA-type applications.

GEArray® Q Series Gene Expression Arrays Protocol for Odyssey v8344 - LI-COR Biosciences (9/2008)
GEArray Q Series Gene Expression Arrays Protocol, superarray, super array

In-Cell Western Assay Kit I and Kit II v8911 - LI-COR Biosciences (7/2010)
Protocol for In-Cell Western Assay Kit I or Kit II

IRDye® Peptide Labeling Application Guide v1.0 - LI-COR (3/2009)
IRDye Peptide Labeling Application Guide

Near-Infrared Fluorescent β-galactosidase Reporter Gene Assays Using DDAOG v1.0 - LI-COR Biosciences (8/2009)
This protocol describes a sensitive β-gal activity assay that uses the DDAOG substrate, 9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl) β-D-galactopyranoside, to generate a near-infrared fluorescent signal that can be detected with the Odyssey® Infrared Imaging System.

Odyssey Application Protocols Manual v09546 - LI-COR Biosciences (10/2009)
Complete Odyssey protocols manual with all protocols.

Odyssey ICW Assay: Apoptosis Assay Example Detailing the Seeding, Induction, and Detection of the HeLa Cellular Response to Anisomycin Treatment v8338 - LI-COR Biosciences (7/2010)
ICW protocol for the quantitative determination of apoptosis (cleaved caspase-3) in HeLa cells in response to Anisomycin treatment.

Odyssey ICW Assay: Measuring IC50 of Inhibitor PD168393 in A431 Cells Responding to Epidermal Growth Factor v8599 - LI-COR Biosciences (7/2010)
ICW protocol for the IC50 determination of the tyrosine kinase inhibitor PD168393 on multiple targets simultaneously

Odyssey ICW Assay: Measuring IC50 of Inhibitor U0126 in NIH3T3 Responding to Acidic Fibroblast Growth Factor (aFGF-1) v8334 - LI-COR Biosciences (7/2010)
ICW protocol for the IC50 determination of the MEK inhibitor U0126 on NIH3T3 cells

Odyssey ICW Assay: Nucleic Acid Stains (TO-PRO-3 and Syto 60) Used as Normalizing Agents v8342 - LI-COR Biosciences (7/2010)
Protocol for signal normalization using DNA content with nucleic acid-specific dyes

Odyssey ICW Assay: Phospho-p38 Detection in HeLa Cells in Response to Anisomycin v9368 - LI-COR Biosciences (7/2010)
In-Cell Western Assay for Phospho-p38 Detection in HeLa Cells in Response to Anisomycin

Odyssey ICW Assay: Phospho-p53 Detection in COS Cells in Response to Hydroxyurea v8339 - LI-COR Biosciences (7/2010)
ICW protocol for the quantitative determination of p53 phosphorylation in COS cells in response to stimulation with hydroxyurea

Odyssey ICW Assay: PMA-induced ERK Activation in Suspension Cell Lines v8340 - LI-COR Biosciences (7/2010)
Odyssey ICW sample protocol for PMA-induced ERK Activation in Suspension Cell Lines

Odyssey ICW Assay: Seeding Stimulation, and Detection of NIH3T3 Cellular Response to Acidic Fibroblast Growth Factor (aFGF-1) v8337 - LI-COR Biosciences (7/2010)
ICW protocol for the quantitative determination of ERK phosphorylation in NIH3T3 cells in response to aFGF-1 stimulation.

Odyssey ICW Assay: Seeding Stimulation, and Detection of NIH3T3 Cellular Response to Platelet Derived Growth Factor BB (PDGF-BB) v8336 - LI-COR Biosciences (7/2010)
ICW protocol for the quantitative determination of AKT phosphorylation in NIH3T3 cells in response to PDGF-BB stimulation.

Odyssey ICW Assay: Seeding Stimulation, and Detection of the HeLa Cellular Response to Epidermal Growth Factor v8335 - LI-COR Biosciences (7/2010)
ICW protocol for the quantitative determination ERK phosphorylation in HeLa cells in response to EGF stimulation.

Odyssey ICW Assays: FAQs For Suspension Cells v8341 - LI-COR Biosciences (9/2008)
FAQs for Odyssey In-Cell Westerns and suspension cells.

Odyssey In Cell Western Assay-Sample Protocol for PMA-induced ERK Activation in Suspension Cell Lines v8340 - LI-COR Biosciences (9/2008)
Sample protocol with data samples of PMA-induced ERK activation in suspension cell lines.

Odyssey In-Cell Western Assay: Assessing the Response of A431 Cells to Stimulation with Epidermal Growth Factor v2.0 - LI-COR Biosciences (7/2010)
ICW protocol for the quantitative determination of EGF receptor and ERK phosphorylation in A431 cells.

Odyssey In-Gel Western Detection Protocol v8329 - LI-COR Biosciences (9/2008)
Protocol for in-gel detection of protein targets without blotting

Odyssey Northern Blot Analysis Protocol v9394 - LI-COR Biosciences (9/2008)
Northern blot protocol using biotinylated probes

Odyssey Protocol: Active Motif TransAM™ Transcription Factor Assays v8346 - LI-COR Biosciences (9/2008)
Detailed protocol for adapting Active Motif TransAM assays for detection with the Odyssey System.

Odyssey Protocol: Adapting Panomics TranSignal Cytokine Antibody Arrays v8347 - LI-COR Biosciences (9/2008)
Detailed protocol for adapting Panomics TransSignal Arrays for detection using the Odyssey System

Odyssey Protocol: Cartesian Array™ Human Cytokine Set v8368 - LI-COR Biosciences (9/2008)
Detailed protocol for adapting Biosource Cartesian Array for detection using the Odyssey System

Odyssey Protocol: RayBio Cytokine Antibody Arrays v09742 - LI-COR (12/2008)
Detailed protocol for adapting RayBio Cytokine Antibody Arrays for detection using the Odyssey System

Odyssey Protocol: Tips for Antibody and Lysate Arraying v8014 - LI-COR Biosciences (9/2008)
Tips for preparing antibody and lysate (Reverse Phase) arrays.

Odyssey Quick Card for Western Blots PN 988-09887 v1.0 - LI-COR (11/2008)
Overview quick card for doing Western Blots on the Odyssey System.

Odyssey Southern Blot Detection Using Biotin/Streptavidin v9395.0 - LI-COR Biosciences (9/2008)
Odyssey protocol detailing the Biotin/Streptavidin Southern method.

Odyssey Western Blot Kit Protocol v2.0 - LI-COR (6/2010)
Western Blot Protocol for Odyssey Western Kits

On-Cell Western Plate-Based Assay for Targeted NIR Labeled Optical Imaging Agent Development v1.0 - LI-COR (7/2010)
An application note illustrating the process for testing a particular cell type with the IRDye 800CW EGF for eventual use in vivo.

Protein Array Instructions for VP 478 Reading Mat v1.0 - Vendor (9/2008)
Excel file, instructions and color guides for printing, loading order and reading mats using the LI-COR Protein Arrayer System.

Sigma Panorama Mouse/Rat Tissue Extract Protein Array Detection on Odyssey v8990 - LI-COR Biosciences (9/2008)
Tips for detecting the Panorama Mouse/Rat Tissue Extract Protein Array on the Odyssey System.

Smart Gel™ Protocol v1.0 - LI-COR (11/2009)
Pack insert for Smart Gel. Smart Gel is a novel, ready-to-pour SDS polyacrylamide solution that polymerizes into a unique support matrix for the electrophoretic separation of denatured proteins. The proprietary chemistry of Smart Gel™ eliminates the need for a stacking gel and provides ultra-fine band resolution over a wide molecular weight range.

Super Array Protocol for Odyssey v8344 - LI-COR Biosciences (9/2008)
Protocol for adapting Super Arrays/gene expression arrays on the Odyssey.

Syto® 60 Staining of Nucleic Acids in Gels v1.0 - LI-COR (6/2010)
Nucleic acids stained with the Syto 60 stain can be detected and quantified on the Odyssey® Infrared and Odyssey Fc Imaging Systems using the 700 nm channel.

TranSignal Cytokine Antibody Arrays on the Odyssey System v8350 - LI-COR Biosciences (9/2008)
Cytokine antibody arrays on the Odyssey System

Western Blotting Methods v9288 - LI-COR (9/2008)
Detailed protocol for Western Blot detection in the Odyssey Imaging System. Updated July 2007 with Newblot Stipping Buffers