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Found 42 documents.

A Microsatellite Linkage Map of the European Sea Bass v1.0 - Dimitry A. Chistiakov, Bart Hellemans, Chris S. Haley, Andy S. Law, Costas S. Tsigenopoulos, Georgios Kotoulas, Daniela Bertotta, Angelo Libertini and Filip A. M. Volckaert (4/2009)
A genetic linkage map of the European sea bass (Dicentrarchus labrax) was constructed from 174 microsatellite markers, including 145 new markers reported in this study. The mapping panel was derived from farmed sea bass from the North Adriatic Sea and consisted of a single family including both parents and 50 full-sib progeny (biparental diploids).

AFLP-Based Genetic Linkage Map for the Red Flour Beetle v1.0 - D. Zhong, A. Pai, and G. Yan (4/2009)
The red flour beetle (Tribolium castaneum) is a major pest of stored grain and grain products and a popular model species for a variety of ecological, evolutionary, and developmental biology studies. Development of a linkage map based on reproducible and highly polymorphic molecular markers would greatly facilitate research in these disciplines. The authors have developed a genetic linkage map using 269 amplified fragment length polymorphism (AFLP) markers.

An AFLP-Based Genetic Linkage Map of Channel Catfish (Ictalurus punctatus) Constructed by Using an Interspecific Hybrid Resource Family v1.0 - Zhanjiang Liu, Attila Karsi, Ping Li, Dongfeng Cao, and R. Dunham (4/2009)
Catfish is the major aquaculture species in the United States. The hybrid catfish produced by crossing channel catfish females with blue catfish males exhibit a number of desirable production traits, but their mass production has been difficult. To introduce desirable genes from blue catfish into channel catfish through introgression, a genetic linkage map is helpful. In this project, a genetic linkage map was constructed using amplified fragment length polymorphism (AFLP).

Automated band mapping in electrophoretic gel images using background information v1.0 - Troy Zerr and Steven Henikoff (4/2009)
Some popular methods for polymorphism and mutation discovery involve ascertainment of novel bands by the examination of electrophoretic gel images. Although existing strategies for mapping bands work well for specific applications, such as DNA sequencing, these strategies are not well suited for novel band detection. Here, we describe a general strategy for band mapping that uses background banding patterns to facilitate lane calling and size calibration. We have implemented this strategy in GelBuddy, a user-friendly Java-based program for PC and Macintosh computers, which includes several utilities to assist discovery of mutations and polymorphisms.

Capnocytophaga sp. Isolated from a Cat with Chronic Sinusitis and Rhinitis v1.0 - Erin Frey, Barrak Pressler, James Guy, Christian Pitulle, and Edward Breitschwerdt (4/2009)
A Capnocytophaga sp. was inadvertently isolated from a cat with chronic sinusitis and rhinitis when cytopathic effects were observed in Crandall-Reese feline kidney cells that had been inoculated with oropharyngeal swab samples. Although Capnocytophaga spp. are of considerable zoonotic importance, their clinical relevance for dogs or cats has not been established. However, failure to do so may be attributed to the infrequent use of specialized isolation techniques that are required to grow Capnocytophaga spp. To our knowledge, successful isolation of these organisms from a cat with nasopharyngeal disease has not been reported.

Characterization of Bacterial Community Diversity in Cystic Fibrosis Lung Infections by Use of 16S Ribosomal DNA Terminal Restriction Fragment Length Polymorphism Profiling v1.0 - G. B. Rogers, M. P. Carroll, D. J. Serisier, P. M. Hockey, G. Jones, and K. D. Bruce (4/2009)
Progressive loss of lung function resulting from the inflammatory response to bacterial colonization is the leading cause of mortality in cystic fibrosis (CF) patients. A greater understanding of these bacterial infections is needed to improve lung disease management. As culture-based diagnoses are associated with fundamental drawbacks, we used terminal restriction fragment (T-RF) length polymorphism profiling and 16S rRNA clone data to characterize, without prior cultivation, the bacterial community in 71 sputa from 34 adult CF patients. (Note: Please contact author for more information).

Determination of Genetic Diversity in Tall Fescue with AFLP Markers v1.0 - M. A. Rouf Mian, Andrew A. Hopkins, and John C. Zwonitzer (4/2009)
Little information is available regarding genetic variation in tall reprofescue (Festuca arundinacea Schreb). Such information is important in constructing mapping populations and targeting germplasm collec- marktion and utilization. The objective of this study was to evaluate the genetic diversity among a number of tall fescue accessions, cultivars, and selected genotypes. In one experiment, 16 persistent tall fescue plants (genotypes) were assayed by a fluorescence-labeled amplified fragment length polymorphism (AFLP) detection method usingDNA samples from individual plants.

Discovery of induced point mutations in maize genes by TILLING v1.0 - Steve Henikoff, Cliff Weil (9/2008)
The authors demonstrate that high-throughput TILLING is applicable to maize, an important crop plant with a large genome but with limited reverse-genetic resources currently available. They screened pools of DNA samples for mutations in 1-kb segments from 11 different genes, obtaining 17 independent induced mutations from a population of 750 pollen-mutagenized maize plants. One of the genes targeted was the DMT102 chromomethylase gene, for which they obtained an allelic series of three missense mutations that are predicted to be strongly deleterious.

Efficient Target-Selected Mutagenesis in Zebrafish v1.0 - Erno Wienholds, Freek van Eeden, Marit Kosters, Josine Mudde, Ronald H.A. Plasterk, and Edwin Cuppen (4/2009)
One of the most powerful methods available to assign function to a gene is to inactivate or knockout the gene. Recently,we described the first target-selected knockout in zebrafish. Here,we report on the further improvements of this procedure,resulting in a highly efficient and easy method to do target-selected mutagenesis in zebrafish.

Empirical Evaluation of Genetic Clustering Methods Using Multilocus Genotypes From 20 Chicken Breeds v1.0 - Noah A. Rosenberg, Terry Burke, Kari Elo, Marcus W. Feldman, Paul J. Freidlin, Martien A. M. Groenen, Jossi Hillel, Asko Mäki-Tanila, Michèle Tixier - Boichard, Alain Vignal, Klaus Wimmers, and Steffen Weigend (4/2009)
The authors tested the utility of genetic cluster analysis in ascertaining population structure of a large data set for which population structure was previously known. Each of 600 individuals representing 20 distinct chicken breeds was genotyped for 27 microsatellite loci, and individual multilocus genotypes were used to infer genetic clusters.

Evidence for fine-scale genetic structure and estuarine colonisation in a potential high gene flow marine goby (Pomatoschistus minutus) v1.0 - C Pampoulie, ES Gysels, GE Maes, B Hellemans, V Leentjes, AG Jones and FAM Volckaert (4/2009)
Marine fish seem to experience evolutionary processes that are expected to produce genetically homogeneous populations. We have assessed genetic diversity and differentiation in 15 samples of the sand goby Pomatoschistus minutus (Pallas, 1770) (Gobiidae, Teleostei) from four major habitats within the Southern Bight of the North Sea, using seven microsatellite and 13 allozyme loci.

Evidence of a new leukemia inhibitory factor-associated genetic marker for litter size in a synthetic pig line v1.0 - A. Spötter, C. Drögemüller, H. Hamann and O. Distl (4/2009)
The association of a diallelic polymorphism in the leukemia inhibitory factor (LIF) gene with reproductive, growth, and carcass traits was studied in a German synthetic pig line. The diallelic SNP has been located in the 3′-untranslated region of the third exon of the porcine LIF gene. Information on 955 litter records from 273 genotyped sows was used in the analyses with respect to the number of piglets born alive.

Evolution of Repeated Sequence Arrays in the D-Loop Region of Bat Mitochondrial DNA v1.0 - Gerald S. Wilkinson, Frieder Mayer, Gerald Kerth and Barbara Petri (4/2009)
Analysis of mitochondrial DNA control region sequences from 41 species of bats representing 11 families revealed that repeated sequence arrays near thet RNA-Pro gene are present ainll vespertilionine bats.

Evolution of Replication Efficiency following Infection with a Molecularly Cloned Feline Immunodeficiency Virus of Low Virulence v1.0 - Margaret J. Hosie, Brian J. Willett, Dieter Klein, Thomas H. Dunsford, Celia Cannon, Masayuki Shimojima, James C. Neil, and Oswald Jarrett (4/2009)
The development of an effective vaccine against human immunodeficiency virus is considered to be the most practicable means of controlling the advancing global AIDS epidemic. Studies with the domestic cat have demonstrated that vaccinal immunity to infection can be induced against feline immunodeficiency virus (FIV); however, protection is largely restricted to laboratory strains of FIV and does not extend to primary strains of the virus.

Fluorescent Amplified Fragment Length Polymorphism Probabilistic Database for Identification of Bacterial Isolates from Urinary Tract Infections v1.0 - Yankuba Kassama, Paul J. Rooney, and Royston Goodacre (4/2009)
The ability of the fluorescent amplified fragment length polymorphism (FAFLP) technique to identify bacterial isolates from urinary tract infections (UTIs) was investigated.

Genetic Analysis of Phenoxyalkanoic Acid Degradation in Sphingomonas herbicidovorans MH v1.0 - Tina A. Müller, Steven M. Byrde, Christoph Werlen, Jan Roelef van der Meer, and Hans-Peter E. Kohler (4/2009)
Phenoxyalkanoic acid degradation is well studied in Beta- and Gammaproteobacteria, but the genetic background has not been elucidated so far in Alphaproteobacteria. We report the isolation of several genes involved in dichlor- and mecoprop degradation from the alphaproteobacterium Sphingomonas herbicidovorans MH and propose that the degradation proceeds analogously to that previously reported for 2,4-dichlorophenoxyacetic acid (2,4-D).

Genetic Differentiation of Tetraploid Creeping Bentgrass and Hexaploid Redtop Bentgrass Genotypes by AFLP and their Use in Turfgrass Breeding v1.0 - Georgina V. Vergara and Suleiman S. Bughrara (4/2009)
The turf industry in the last decade has seen doubling in number of new creeping bentgrass [Agrostis stolonifera var. palustris (Huds.)Farw. and A. stolonifera var. stolonifera Huds.] cultivars, many with unknown variability and lineage. Understanding the genetic diversity of putative parental and wild stocks would be useful in plant breeding programs. Amplified fragment length polymorphism (AFLP) analysis was conducted to investigate genetic variability among old and new cultivars of creeping bentgrasses, redtop bentgrasses (Agrostis gigantea Roth), plant introductions, and selected creeping bentgrass genotypes with resistance to gray snow mold (Typhula incarnata Lasch).

Genetic Effects on Age-Dependent Onset and Islet Cell Autoantibody Markers in Type 1 Diabetes v1.0 - Jinko Graham, William A. Hagopian, Ingrid Kockum, Lou Shend Li, Carani B. Sanjeevi, Robert M. Lowe, Jonathan B. Schaefer, Marjan Zarghami, Heather L. Day, Mona Landin-Olsson, Jerry P. Palmer, Marta Janer-Villanueva, Leroy Hood, Göran Sundkvist, Åke Lernmark, Norman Breslow, Gisela Dahlquist and Göran Blohmé (4/2009)
Age-dependent associations between type 1 diabetes risk genes HLA, INS VNTR, and CTLA-4 and autoantibodies to GAD65 (GADAs), ICA512/IA-2, insulin, and islet cells were determined by logistic regression analysis in 971 incident patients with type 1 diabetes and 702 control subjects aged 0–34 years. GADAs were associated with HLA-DQ2 in young but not in older patients (P 0.009). Autoantibodies to insulin were negatively associated with age (P < 0.0001) but positively associated with DQ8 (P 0.03) and with INS VNTR (P 0.04), supporting possible immune tolerance induction. ICA512/IA-2 were negatively associated with age (P < 0.0001) and with DQ2 (P < 0.0001) but positively associated with DQ8 (P 0.04). Males were more likely than females to be negative for GADA (P < 0.0001), autoantibodies to islet cells (P 0.04), and all four autoantibody markers (P 0.004). The CTLA-4 3 end microsatellite marker was not associated with any of the autoantibodies.

Genetic Relationships of Aglaonema Species and Cultivars Inferred from AFLP markers v1.0 - Jianjun Chen, Pachanoor S. Devanand, David J. Norman, Richard J. Henny, Chih-Cheng T. Chao (4/2009)
Aglaonema is an important ornamental foliage plant genus, but genetic relationships among its species and cultivars have not been reported. This study analysed genetic relatedness of 54 cultivars derived from nine species using ampli®ed fragment length polymorphism (AFLP) markers.

Genetic Variation in Coding Regions Between and Within Commonly Used Inbred Rat Strains v2.0 - Bart M.G. Smits, Bert F.M. van Zutphen, Ronald H.A. Pasterk and Edwin Cuppen (4/2009)
Single nucleotide polymorphisms (SNPs) are the most common genetic variation in mammalian populations. Their significance is illustrated by their potential contribution to common disease but also by their potential for use in genetic association and mapping experiments. The authors have examined the genetic variation between commonly used inbred rat strains by using an efficient SNP discovery and typing assay based on enzyme-based (CEL I) heteroduplex cleavage.

Genomic Mapping of Direct and Correlated Responses to Long-Term Selection for Rapid Growth Rate in Mice v1.0 - Mark F. Allan, Eugene J. Eisen and Daniel Pomp (4/2009)
Understanding the genetic architecture of traits such as growth, body composition, and energy balance has become a primary focus for biomedical and agricultural research. The objective of this study was to map QTL in a large F2 (n 1181) population resulting from an intercross between the M16 and ICR lines of mice.

High-Density Linkage Mapping Revealed Suppression of Recombination at the sex determination locus in Papaya v1.0 - Hao Ma, Paul H. Moore, Zhiyong Liu, Minna S. Kim, Qingyi Yu, Maureen M. M. Fitch, Terry Sekioka, Andrew H. Paterson and Ray Ming (4/2009)
A high-density genetic map of papaya (Carica papaya L.) was constructed using 54 F2 plants derived from cultivars Kapoho and SunUp with 1501 markers, including 1498 amplified fragment length polymorphism (AFLP) markers, the papaya ringspot virus coat protein marker, morphological sex type, and fruit flesh color.

High-throughput discovery of rare human nucleotide polymorphisms by Ecotilling v1.0 - Steve Henikoff (9/2008)
Human individuals differ from one another at ~0.1% of nucleotide positions, but these single nucleotide differences account for most heritable phenotypic variation. Large-scale efforts to discover and genotype human variation have been limited to common polymorphisms. However, these efforts overlook rare nucleotide changes that may contribute to phenotypic diversity and genetic disorders, including cancer. Thus, there is an increasing need for high-throughput methods to robustly detect rare nucleotide differences. This publication discusses adaptation of the mismatch discovery method known as Ecotilling to discovery of human single nucleotide polymorphisms. A universal primer strategy and implemented algorithms for automated band detection are discussed for reducing costs. Ecotilling was validated by screening 90 human DNA samples for nucleotide changes in 5 gene targets and by comparing results to public resequencing data. Ecotilling was shown to be efficient relative to resequencing, with a false negative rate of 5% and a false discovery rate of 4%. Twenty eight new rare alleles were found, including some that are predicted to damage protein function. The detection of rare damaging mutations has implications for models of human disease.

High-Throughput Screening for Induced Point Mutations v1.0 - Trenton Colbert, Bradley J. Till, Rachel Tompa, Steve Reynolds, Michael N. Steine, Anthony T. Yeung, Claire M. McCallum, Luca Comai, and Steven Henikoff (4/2009)
With the completion of genome sequencing projects, emphasis in genomics has shifted from analyzing sequences to understanding gene function, and effective reverse genetic strategies are increasingly in demand. Here we report adaptations of the targeting induced local lesions in genomes (TILLING) reverse genetic strategy (McCallum et al., 2000a) to make it suitable for large-scale screening of chemically induced mutations in Arabidopsis and other plants.

Human Herpesvirus 7 Open Reading Frame U12 Encodes a Functional Chemokine Receptor v1.0 - Kazuski Nakano, Kenjiro Tadagaki, Yuji Isegawa, Mya Mya Aye, Ping Zou, and Koichi Yamanishi (4/2009)
Human herpesvirus 7 (HHV-7), which belongs to the betaherpesvirus subfamily, infects mainly CD4 T cells in vitro and infects children during infancy. After the primary infection, HHV-7 becomes latent. HHV-7 contains two genes (U12 and U51) that encode putative homologs of cellular G-protein-coupled receptors. To analyze the biological function of the U12 gene, we cloned the gene and expressed the U12 protein in cells.

Identification and Characterization of Simple Sequence Repeat Markers in Alpaca v1.0 - Geneseek (9/2008)
Simple-sequence-repeat (SSR) or microsatellite markers have been used extensively in genome mapping, parentage verification, population structure and comparable genome analysis. The Alpaca Registry was an early adopter of DNA testing and has one of the most rigorously documented stud books. More than 85,000 alpaca have been genotyped and their parentage verified using a 10-SSR marker panel since 1998. The number of alleles per marker ranges from 6 to 28. Prominent alleles within a locus remain virtually the same for all markers when analysis was conducted using 2 subsets of data generated by different labs over time. As line breeding and inbreeding take place, closely related animals will become increasingly difficult to differentiate using the existing marker panel. Several new informative SSR markers have been identified, 5 of which are now included in routine alpaca parentage analysis.

Identification of Amplified Restriction Fragment Length Polymorphism Markers Linked to Genes Controlling Boar Sperm Viability Following Cryopreservation v1.0 - Lisa M. Thurston, Ken Siggins, Alan J. Mileham, Paul F. Watson, and William V. Holt (4/2009)
This study investigated two hypotheses: 1) that consistent between- boar variation in frozen semen quality exists and is genetically determined, and 2) molecular markers linked to genes controlling semen freezability can be identified using amplified restriction fragment length polymorphism (AFLP) technology.

Identification of Canadian durum wheat varieties using a single PCR v1.0 - Daniel Perry (9/2008)
Accurate and reliable means of variety identification are necessary to assess purity of seed supplies, to support claims relating to plant breeders’ rights and, in Canada, to provide quality assurances in the grain handling system. A single, multiplexed set of seven simple-sequence-repeat (SSR) markers was found to uniquely identify all 18 durum wheat varieties that have been developed in Canada and are currently, or were formerly, registered for commercial production. Significant features of this multiplexed set include an allele that is specific, within Canadian durum varieties, to those having high gluten strength, and redundancy that was included in an effort to increase the capacity to accommodate future varieties. In combination with a reasonably rapid individual-kernel DNA extraction protocol and automated allele calling, this marker system offers a higher resolution alternative to complement established protein-based variety identification methods.

Identification of quantitative trait loci affecting reproduction in pigs v1.0 - J. P. Cassady, R. K. Johnson, D. Pomp, G. A. Rohrer, L. D. Van Vleck, E. K. Spiegel, and K. M. Gilson (4/2009)
The objective of this research was to identify chromosomal regions harboring QTL affecting reproduction in pigs.

Inbreeding levels of two Ustilago maydis populations v1.0 - Charles W. Barnes, Les J. Szabo, Georgiana May, and James V. Groth (4/2009)
Little is known about the population mating behavior of the smut fungus Ustilago maydis DC (Corda). To determine the amount of inbreeding that occurs in local U. maydis populations, two cornfields were sampled, one in North America (NA) at Le Sueur, Minnesota, and one in South America (SA) at Tarariras, Uruguay. These fields were chosen because of their geographic isolation and host management differences.

Linkage and Association Mapping of a Chromosome 1q21-q24 Type 2 Diabetes Susceptibility Locus in Northern European Caucasians v1.0 - Swapan Kumar Das, Sandra J. Hasstedt, Zhengxian Zhang, and Steven C. Elbein (4/2009)
We have identified a region on chromosome 1q21-q24 that was significantly linked to type 2 diabetes in multiplex families of Northern European ancestry and also in Pima Indians, Amish families, and families from France and England. We sought to narrow and map this locus using a combination of linkage and association approaches by typing microsatellite markers at 1.2 and 0.5 cM densities, respectively, over a region of 37 cM (23.5 Mb).

Microsatellite marker development and analysis in the Eastern Oyster (Crassostrea virginica): Confirmation of null alleles and non-mendelian segregation ratios v1.0 - K. S. Reece, W. L. Ribeiro, P. M. Gaffney, R. B. Carnegie, and S. K. Allen, Jr. (4/2009)
Eighteen microsatellite markers were developed for the Crassostrea virginica nuclear genome, including di-, tri-, and tetranucleotide microsatellite repeat regions that included perfect, imperfect, and compound repeat sequences.

Molecular Characterization of Multidrug-Resistant Salmonella enterica subsp. enterica Serovar Typhimurium Isolates from Swine v1.0 - Wondwossen Abebe Gebreyes and Craig Altier (4/2009)
As part of a longitudinal study of antimicrobial resistance among salmonellae isolated from swine, we studied 484 Salmonella enterica subsp. enterica serovar Typhimurium (including serovar Typhimurium var. Copenhagen) isolates. We found two common pentaresistant phenotypes.

Mutations in the 59 Nontranslated Region of Bovine Viral Diarrhea Virus Result in Altered Growth Characteristics v1.0 - Paul Becher, Michaela Orlich and Heinz-Jürgen Thiel (4/2009)
The 5* nontranslated region (NTR) of pestiviruses functions as an internal ribosome entry site (IRES) that mediates cap-independent translation of the viral polyprotein and probably contains additional cis-acting RNA signals involved in crucial processes of the viral life cycle. Computer modeling suggests that the 5*-terminal 75 nucleotides preceding the IRES element form two stable hairpins, Ia and Ib. Spontaneous and engineered mutations located in the genomic region comprising Ia and Ib were characterized by using infectious cDNA clones of bovine viral diarrhea virus.

Nuclease Enzyme Options v1.0 - Bradley J. Till, Chris Burtner, Luca Comai and Steven Henikoff (4/2009)
This is the publication that discusses the nuclease enzyme options for TILLING/Ecotilling.

Panmixia in the European eel: a matter of time. v1.0 - Johan Dannewitz, Gregory E. Maes, Leif Johansson, Håkan Wickström, Filip A. M. Volckaert and Torbjörn Järvi (4/2009)
The European eel (Anguilla anguilla L.) has been a prime example of the panmixia paradigm because of its extraordinary adaptation to the North Atlantic gyral system, semelparous spawning in the Sargasso Sea and long trans-oceanic migration.

Paternity and paternal effort in the pumpkinseed sunfish v1.0 - Oscar Rios-Cardenas and Michael S. Webster (4/2009)
Theoretical models suggest that males should adjust their parental effort according to paternity when parental effort is costly, paternity varies among clutches, and males have a cue to assess paternity.

pmoA-Based Analysis of Methanotrophs in a Littoral Lake Sediment Reveals a Diverse and Stable Community in a Dynamic Environment v1.0 - Michael Pester, Michael W. Friedrich, Bernhard Schink, and Andreas Brune (4/2009)
Diversity and community structure of aerobic methane-oxidizing bacteria in the littoral sediment of Lake Constance was investigated by cloning analysis and terminal restriction fragment length polymorphism (T-RFLP) fingerprinting of the pmoA gene.

Poxvirus Orthologous Clusters: toward Defining the Minimum Essential Poxvirus Genome v1.0 - Chris Upton, Stephanie Slack, Arwen L Hunter, Angelika Ehlers, and Rachel L. Roper (4/2009)
We describe the use of these tools to identify necessary annotation and sequencing updates in poxvirus genomes. For example, using POCs, we identified 19 genes that were widely conserved in poxviruses but missing from the vaccinia virus strain Tian Tan 1998 GenBank file. We have reannotated and resequenced fragments of this genome and verified that these genes are conserved in Tian Tan. The results for poxvirus genes and genomes are discussed in light of evolutionary processes.

Target-selected mutant screen by TILLING in Drosophila v1.0 - Sylke Winkler, Anja Schwabedissen, Dana Backasch, Christian Bökel, Claudia Seidel, Stefanie Bönisch, Maximilian Fürthauer, Antje Kuhrs, Laura Cobreros, Michael Brand, and Marcos González-Gaitán (4/2009)
The availability of the full Drosophila genomic DNA sequence prompts the development of a method to efficiently obtain mutations in genes of interest identified by their sequence homologies or biochemically. To date, molecularly characterized mutations have been generated in around 6000 of the ∼15,000 annotated fly genes, of which around one-third are essential for viability. To obtain mutations in essential and nonessential genes of interest, we took a reverse genetics approach, based on the large-scale detection of point mutations by Cel-I-mediated heteroduplex cleavage. A library of genomic DNA from 2086 EMS-mutagenized lines was established. The library was screened for mutations in three genes. A total of 6.1 Mb were screened, and 44 hits were found in two different mutagenesis conditions. Optimal conditions yielded an average of one mutation every 156 kb. For an essential gene tested, five of 25 mutations turned out to cause lethality, confirming that EMS mutagenesis leads to high frequency of gene inactivation. We thereby established that Cel-I-mediated TILLING can be used to efficiently obtain mutations in genes of interest in Drosophila.

Transcriptional Activation of the Human Hematopoietic Prostaglandin D Synthase Gene in Megakaryoblastic Cells v1.0 - Ko Fujimori, Yoshihide Kanaoka, Yuriko Sakaguchi, and Yoshihiro Urade (4/2009)
The human hematopoietic prostaglandin D synthase (H-PGDS) gene is highly expressed in human megakaryoblastic cells, in which phorbol ester induces its expression. The authors characterized the promoter activity of the 5*-flanking region and the untranslated exon 1 (21044 to 1290) of the human H-PGDS gene in human megakaryoblastic Dami cells.

TWO VISUAL PIGMENTS IN A SINGLE PHOTORECEPTOR CELL: IDENTIFICATION AND HISTOLOGICAL LOCALIZATION OF THREE mRNAS ENCODING VISUAL PIGMENT OPSINS IN THE RETINA OF THE BUTTERFLY PAPILIO XUTHUS v1.0 - Junko Kitamoto, Katsuhiko Sakamoto, Koichi Ozaki, Yuji Mishina, and Kentaro Arikawa (4/2009)
This paper describes the localization of newly identified visual pigment opsins in the tiered retina of the Japanese yellow swallowtail Papilio xuthus.