LI-COR, Inc. 4647 Superior Street P.O.Box 4425 Lincoln, NE 68504 USA Phone: (402) 467-0700 Toll Free: (800) 447-3576 Fax: (402) 467-0819 www.licor.com

MATERIAL SAFETY DATA SHEET

1. PRODUCT IDENTIFICATION

5X New Blot™ Nitro Stripping Buffer

2. COMPOSITIONS AND INFORMATION ON INGREDIENTS

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   Chemical Name       CAS#            W/V%       EXPOSURE LIMITS IN AIR-OSHA
   NE = NOT ESTABLISHED                           PEL       STEL       IDLH      OTHER
                                                 mg/m3      mg/m3      mg/m3     mg/m3
COMPONENTS: Buffer Solution
Water                7732-18-5        <95         NE        NE         NE          NE
Proprietary                           <02         2mg/m3    NE         NE          10mg/m3 ACGIH
Proprietary                           <02         NE        NE         NE          NE
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3. HAZARD IDENTIFICATION

Stripping Buffer

Stripping Buffer

Hazard Symbols:
     C
Risk Phrases:
     R 35 Causes severe burns
Safety Phrases:
     S 26/27 In case of contact with eyes, rinse immediately with plenty of
     water and seek medical advice. Take off immediately all contaminated clothing
     S 37/39 Wear suitable gloves and eye/face protection.
     S 45 In case of accident or if you feel unwell, seek medical advice
     immediately (show the label where possible).

Classification system: The classification was made according to the latest editions of the EU lists, and expanded upon from company literature data.

Symptoms of Over-Exposure by Route of Exposure:
Eye: Causes eye irritation. May cause chemical conjunctivitis and corneal damage.
Skin: Causes skin irritation.
Ingestion: May cause gastrointestinal irritation with nausea, vomiting and diarrhea.
Inhalation: May cause respiratory tract irritation.
Chronic: No information found.

4. FIRST AID MEASURES

Eyes: Get medical aid. In case of contact, immediately flush eyes with plen ty of water for at least 15 minutes. Get medical aid.
Skin: In case of contact, flush skin with plenty of water. Remove contaminated clothing and shoes. Get medical aid if irritation develops and persists. Wash clothing before reuse.
Ingestion: If swallowed, do not induce vomiting unless directed to do so by medical personnel. Never give anything by mouth to an unconscious person. Get medical aid.
Inhalation: Remove from exposure and move to fresh air immediately. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical aid.
Notes to Physician: Treat symptomatically and supportively.

5. FIRE-FIGHTING MEASURES

Unusual Fire And Explosion Hazards: When involved in a fire, this material may decompose and produce irritating fumes and toxic gases (including carbon monoxide, carbon dioxide and oxides of nitrogen).
Flash Point, °C: N/A
Auto ignition Temperature, °C: N/A
Flammable Limits (In Air By Volume, %): N/A
Fire Extinguishing Materials: NA

NFPA Rating: (estimated) Health: 2; Flammability: 0; Instability: 0

6. ACCIDENTAL RELEASE MEASURES

Spill And Leak Response: For small releases take basic hygiene precautions.  Gloves, a lab coat, and eye protection should be worn.  Absorb spilled material with vermiculite.  Wash contaminated area with soap and water, absorb with vermiculite and rinse with water.  Trained personnel using pre-planned procedures would respond to large releases that are not immediately controlled.  Proper protection equipment should be used.  In case of a spill, clear the affected area, protect people and respond with trained personnel.

In the event of a non-incidental, minimum release, Personal Protective Equipment should be:

Level D: lab gloves, chemical resistant apron, boots and splashes goggles.  Respiratory protection should not be necessary.  Absorb spilled liquid with poly pads or other suitable absorbent materials.  Decontaminate the area thoroughly.  Place all spill residues in a suitable container and seal.  Dispose of in accordance with Federal, State and local hazardous waste disposal regulation.

7. HANDLING and STORAGE

Work Practices And Hygiene Practices: As with all chemicals, avoid getting this product ON YOU or IN YOU.  Wash hands after handling this product.  Avoid splashing or spraying this product.  Do not ear or drink while handling this product.
Storage And Handling Practices: All employees who handle this material should be trained to handle it safely.  Avoid breathing vapors or mists generated by this product. Ensure that containers of this product are properly labeled.  Open containers slowly on a stable surface.  Store vials as directed in the product insert.  Keep vials tightly closed when not in use.  Store away from incompatible materials.  Inspect vials containing this product for leaks or damage.  Prior to use, read instruction provided with product.
Protective Practices During Maintenance Of Contaminated Equipment:  Follow practices indicated in Section 6 (Accidental Release Measures).  Make certain that application equipment is locked and tagged-out safely, as applicable.  Always use this product in areas where adequate ventilation is provided.  Decontaminate equipment using soapy water before maintenance begins.  Collect all rinsates and dispose of according to applicable Federal, State or Local procedures.

8. EXPOSURE CONTROLS-PERSONAL PROTECTION

Ventilation And Engineering Controls: Use with adequate ventilation.  Use a mechanical fan or vent area to outside, if necessary.  Eye-wash stations should be available near location where his product is used.
Respiratory Protection: Respiratory protection is generally not needed when using this product.  Maintain airborne contaminant concentration below limits listed in Section 2. (Composition and Information on Ingredients).  If respiratory protection is needed, use only protection authorized in 29 CFR 1910.134 or applicable State regulations. Use supplied air respiration protection if oxygen levels are below 19.5% or are unknown.
Eye Protection: Splash goggles or safety glasses.
Hand Protection: Wear butyl rubber gloves for routine industrial use.
Body Protection: Use body protection appropriate for task, such as a lab coat.

9. PHYSICAL and CHEMICAL PROPERTIES

10. STABILITY and REACTIVITY

Stability: Stable under normal room temperature and closed container.
Decomposition Products:Toxic fumes of sodium oxide
Hazardous Polymerization: Will not occur
Conditions To Avoid: Metals and Acids .

11. TOXICOLOGICAL INFORMATION

Toxicity Data:
RTECS#:      
CAS# 7732-18-5: ZC0110000      
CAS# 1310-73-2: WB4900000
LD50/LC50:
CAS# 7732-18-5:
     Oral, rat: LD50 = >90 mL/kg;
CAS# 1310-73-2:
     Draize test, rabbit, eye: 400 ug Mild;
     Draize test, rabbit, eye: 1% Severe;
     Draize test, rabbit, eye: 50 ug/24H Severe;
     Draize test, rabbit, eye: 1 mg/24H Severe;
     Draize test, rabbit, skin: 500 mg/24H Severe;
Carcinogenicity:
CAS# 7732-18-5: Not listed by ACGIH, IARC, NTP, or CA Prop 65.
CAS# 1310-73-2: Not listed by ACGIH, IARC, NTP, or CA Prop 65.
Epidemiology: No information found
Teratogenicity: No information found
Reproductive Effects: No information found
Mutagenicity: No information found
Neurotoxicity: No information found

12. ECOLOGICAL INFORMATION

Environmental Stability: The chemicals in this product's solutions will degrade in the environment into organic and inorganic constituents.
Effect Of Material On Plants Or Animals: No unusual effects on plants or animals are expected if this product is released into the environment.  See Section 11 (Toxicological Information) for further information regarding the effects of chemicals in this product's solutions on test animals.

13. DISPOSAL CONSIDERATIONS

Preparing Wastes For Disposal: Waste Disposal must be in accordance with appropriate Federal, State and local regulation.  This product, if altered by use, may be disposed of by treatment at a permitted facility or as advised by your local hazardous waste regulatory authority.
EPA Waste Number: Not applicable to this product.

14. TRANSPORTATION INFORMATION

This material is hazardous as defined by 49 CFR 172.101 by the U.S. Department of Transportation.  This material is considered as Dangerous Goods.

Please contact LI-COR Biosciences for Transportation Information.

15. REGULATORY INFORMATION

SARA Reporting Requirements: These chemicals are not subject to Sections 302, 304, and 313 reporting requirements under the Superfund Amendment and Reauthorization Act.

Chemical                                               SARA 302                          SARA 304                        SARA 313

SARA Threshold Planning Quantity: N/A
TSCA Inventory Status: N/A

CERCLA Reportable Quantity (RQ): 1000 lbs. (454 Kg) RQ
Other Federal Regulations: N/A

16. OTHER INFORMATION