LI-COR,
Inc.
4647 Superior Street
P.O.Box 4425
Lincoln, NE 68504 USA
Phone: (402)
467-0700
Toll Free: (800) 447-3576
Fax: (402) 467-0819
www.licor.com
|
LI-COR,
Inc. Phone: (402)
467-0700 |
MATERIAL SAFETY DATA SHEET
|
Part No. 830-06258, 830-06194, 830-06195 |
24 Hour Phone (CHEMTREC) (800)424-9300
|
1. PRODUCT IDENTIFICATION
AFLP® 2-Dye Start up Kit
AFLP® IRDye® 700 Startup
AFLP® IRDye® 800 Startup
2. COMPOSITIONS AND INFORMATION ON INGREDIENTS
-------------------------------------------------------------------------------------------------------
Chemical Name CAS# W/V% EXPOSURE LIMITS IN AIR-OSHA
NE = NOT ESTABLISHED PEL STEL IDLH OTHER
mg/m3 mg/m3 mg/m3 mg/m3
COMPONENT 1:Control Template (Genomic Corn DNA)
None of the components in this mixture have an established exposure limit, or contribute significantly
to the hazards associated with product. All hazard information pertinent to this product has been
provided in this Material Safety Data Sheet per the requirements of the OSHA Hazard Communication
Standard (29 CFR 1910.1200)
COMPONENT 2:Mse 1 Primers (containing dNTPs)
None of the components in this mixture have an established exposure limit, or contribute significantly
to the hazards associated with product. All hazard information pertinent to this product has been
provided in this Material Safety Data Sheet per the requirements of the OSHA Hazard Communication
Standard (29 CFR 1910.1200)
COMPONENT 3:IR-labeled Taq 1 Primers
None of the components in this mixture have an established exposure limit, or contribute significantly
to the hazards associated with product. All hazard information pertinent to this product has been
provided in this Material Safety Data Sheet per the requirements of the OSHA Hazard Communication
Standard (29 CFR 1910.1200)
COMPONENT 4:10X Amplification Buffer
Tris-HCl 1185-53-1 <1 NE NE NE NE
Potassium Chloride 7447-40-7 <1 NE NE NE NE
Magnesium Chloride 7786-30-3 <1 NE NE NE NE
Water 7732-18-5 <97 NE NE NE NE
COMPONENT 5:Blue Stop Solution
EDTA 60-00-4 <1 NE NE NE NE
Bromophenol Blue 62625-28-9 <1 NE NE NE NE
Water 7732-18-5 <14 NE NE NE NE
Formamide 75-12-7 <87 30 45 NE 15
(vacated (vacated NIOSH REL
1989 PEL) 1989 PEL) (skin)
20 ppm 30 ppm
COMPONENT 6: Ecor 1/MseI Enzyme Mix
Tris-HCl 1185-53-1 <1 NE NE NE NE
Sodium Chloride 7647-14-5 <1 NE NE NE NE
EDTA 60-00-4 <1 NE NE NE NE
Dithiothreitol 27565-41-9 <1 NE NE NE NE
Bovine serum albumin 9048-46-8 <1 NE NE NE NE
Glycerol 56-81-5 <50 (15 total dust)NE NE NE
Exposure limits are for glycerin mist 5 (respirable fraction)
Vacated 1989 PEL 10 (total)
Triton X-100 9002-93-1 <1 NE NE NE NE
Water 7732-18-5 <45 NE NE NE NE
COMPONENT 7: TaqI Enzyme
TaqI enzyme [1.25 units/µl each in 10 mM Tris-HCl (pH 7.4)] No CAS
Tris-HCl 1185-53-1 <45 NE NE NE NE
Sodium Chloride 7647-14-5 <1 NE NE NE NE
EDTA 60-00-4 <1 NE NE NE NE
Dithiothreitol 27565-41-9 <1 NE NE NE NE
Bovine serum albumin9048-46-8 <1 NE NE NE NE
Glycerol 56-81-5 <50 (15 total dust)NE NE NE
Exposure limits are for glycerin mist 5 (respirable fraction)
Vacated 1989 PEL 10 (total)
Triton X-100 9002-93-1 <1 NE NE NE NE
Bovine serum albumin 9048-46-8 <1 NE NE NE NE
COMPONENT 8: 5x Reaction Buffer
Tris-HCl 1185-53-1 <1 NE NE NE NE
Magnesium acetate 142-72-3 <1 NE NE NE NE
Potassium acetate 127-08-2 <5 NE NE NE NE
Water 7732-18-5 <93 NE NE NE NE
COMPONENT 9: T4 DNA Ligase
Tris-HCl 1185-53-1 <1 NE NE NE NE
Dithiothreitol 27565-41-9 <1 NE NE NE NE
EDTA 6381-92-6 <1 NE NE NE NE
Potassium Chloride 7447-40-7 <1 NE NE NE NE
Bovine serum albumin 9048-46-8 <1 NE NE NE NE
Glycerol 56-81-5 <50 (15 total dust)NE NE NE
Exposure limits are for glycerin mist 5 (respirable fraction)
Vacated 1989 PEL 10 (total)
Water 7732-18-5 <45 NE NE NE NE
COMPONENT 10: Adapter mix
TaqI/MseI Adapters, 1.1 mM ATP, 10 mM Tris-acetate (pH 7.5) No CAS
Tris-HCl 1185-53-1 <10 NE NE NE NE
Magnesium acetate 142-72-3 <10 NE NE NE NE
Potassium acetate 127-08-2 <10 NE NE NE NE
Water 7732-18-5 <70 NE NE NE NE
COMPONENT 11: TE Buffer
Tris-HCl 1185-53-1 <15 NE NE NE NE
EDTA 6381-92-6 <15 NE NE NE NE
Water 7732-18-5 <70 NE NE NE NE
COMPONENT 12:Maize DNA
None of the components in this mixture have an established exposure limit, or contribute significantly
to the hazards associated with product. All hazard information pertinent to this product has been
provided in this Material Safety Data Sheet per the requirements of the OSHA Hazard Communication
Standard (29 CFR 1910.1200)
COMPONENT 13: Pre-amp Primer Mix
None of the components in this mixture have an established exposure limit, or contribute significantly
to the hazards associated with product. All hazard information pertinent to this product has been
provided in this Material Safety Data Sheet per the requirements of the OSHA Hazard Communication
Standard (29 CFR 1910.1200)
COMPONENT 14: Water, Deionized
Water 7732-18-5 100 NE NE NE NE
-------------------------------------------------------------------------------------------------------
3. HAZARD IDENTIFICATION
Component 5-Blue Stop Solution
Hazard Description: T-Toxic
Information pertaining to particular dangers for man and environment.
Note: Substances slightly hazardous to water and ground water.
Risk Phrases:
R 61 May cause harm to the unborn child.
Classification system: The classification was made according to the latest editions of the EU lists, and expanded upon from company and literature data.
Safety Phrases:
S 24/25 Avoid contact with skin and eyes.
S 26 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
S 37 Wear suitable gloves.
S 45 In case of accident or if you feel unwell seek medical advice immediately (show the label where possible).
S 53 Avoid exposure-obtain special instructions before use.
Emergency Overview: None
of the components in this mixture have an established exposure limit,
or contribute significantly to the hazards associated with the product.
All hazard information pertinent to this product has been provided in
the Material Safety Data Sheet per requirements of the OSHA Hazard Communication
Standard (29 CFR 1910.1200). |
Symptoms of Over-Exposure by Route of Exposure: No major adverse health effects should occur from routine occupation use of this material in the manner specified by the manufacturer's instruction. The only likely symptom of exposure would be reddening or inflammation after accidental injection.
4. FIRST AID MEASURES
Skin Exposure: Basic hygiene should prevent
any problems. If contact with this product leads to reddening, inflammation
or irritation, flush the exposed area with running water. Remove any contaminated
clothing, taking care not to contaminate eyes.
Eye Exposure: If liquids or vapors of this product's components
enter the eyes, open victim's eyes while under gently running water. Use
sufficient force to open eyelids. Have victim "roll" eyes.
Minimum flushing is for 15 minutes.
Inhalation: If vapors, mists or sprays of the products components
are inhaled, causing irritation, remove victims to fresh air. If necessary,
use artificial respiration to support vital functions. Remove or cover
gross contamination to avoid exposure to rescuers.
Ingestion: If this product is swallowed, CALL PHYSICIAN OR
POISON CONTROL CENTER FOR MOST CURRENT INFORMATION. If professional advice
is not available, do not induce vomiting. Victim should drink milk, egg
whites, or large quantities of water. Never induce vomiting or give diluents
(milk or water) to someone who is unconscious, having convulsion, or unable
to swallow.
Victims of chemical exposure must be taken for medical attention. Rescuers should be taken for medical attention, if necessary. Take copy of label and MSDS to physician or health professional with victim.
5. FIRE-FIGHTING MEASURES
Unusual Fire And Explosion Hazards: When
involved in a fire, this material may decompose and produce irritating fumes
and toxic gases (including carbon monoxide, carbon dioxide and oxides of nitrogen).
Flash Point, °C: N/A
Auto ignition Temperature, °C: N/A
Flammable Limits (In Air By Volume, %): N/A
Fire Extinguishing Materials: Use suppression methods for surrounding
materials.
6. ACCIDENTAL RELEASE MEASURES
Spill And Leak Response: For small releases, treat the product as water, but take basic hygiene precautions. Lightweight gloves, a lab coat, and eye protection should be worn. Absorb spilled material with paper towels. Wash contaminated area with soap and water, absorb with paper towels and rinse with water. Trained personnel using pre-planned procedures would respond to large releases that are not immediately controlled. Proper protection equipment should be used. In case of a spill, clear the affected area, protect people and respond with trained personnel.
In the event of a non-incidental, minimum release, Personal Protective Equipment should be:
Level D: lab gloves, chemical resistant apron, boots and splashes goggles. Respiratory protection should not be necessary. Absorb spilled liquid with poly pads or other suitable absorbent materials. Decontaminate the area thoroughly. Place all spill residues in a suitable container and seal. Dispose of in accordance with Federal, State and local hazardous waste disposal regulation.
7. HANDLING and STORAGE
Work Practices And Hygiene Practices: As
with all chemicals, avoid getting this product ON YOU or IN YOU. Wash
hands after handling this product. Avoid splashing or spraying this product.
Do not ear or drink while handling this product.
Storage And Handling Practices: All employees who handle this
material should be trained to handle it safely. Avoid breathing vapors
or mists generated by this product. Ensure that containers of this product are
properly labeled. Open containers slowly on a stable surface. Store
vials as directed in the product insert. Keep vials tightly closed when
not in use. Store away from incompatible materials. Inspect vials
containing this product for leaks or damage. Prior to use, read instruction
provided with product.
Protective Practices During Maintenance Of Contaminated Equipment:
Follow practices indicated in Section 6 (Accidental Release Measures).
Make certain that application equipment is locked and tagged-out safely, as
applicable. Always use this product in areas where adequate ventilation
is provided. Decontaminate equipment using soapy water before maintenance
begins. Collect all rinsates and dispose of according to applicable Federal,
State or Local procedures.
8. EXPOSURE CONTROLS-PERSONAL PROTECTION
Ventilation And Engineering Controls: Use
with adequate ventilation. Use a mechanical fan or vent area to outside,
if necessary. Eye-wash stations should be available near location where
his product is used.
Respiratory Protection: Respiratory protection is generally
not needed when using this product. Maintain airborne contaminant concentration
below limits listed in Section 2. (Composition and Information on Ingredients).
If respiratory protection is needed, use only protection authorized in 29 CFR
1910.134 or applicable State regulations. Use supplied air respiration protection
if oxygen levels are below 19.5% or are unknown.
Eye Protection: Splash goggles or safety glasses.
Hand Protection: Wear butyl rubber gloves for routine industrial
use.
Body Protection: Use body protection appropriate for task,
such as a lab coat.
9. PHYSICAL and CHEMICAL PROPERTIES
| Relative Vapor Density
(Air =1): N/A Specific Gravity (Water=1): N/A Solubility In Water: Soluble Appearance And Color: Clear, the STOP solution is blue. How To Detect This Substance: There are no unusual warning properties associate with these solutions. |
Evaporation Rate:
Similar to water Freezing/melting Point: N/A Boiling Point: N/A Ph: 6-8 |
10. STABILITY and REACTIVITY
Stability: Stable
Decomposition Products: Carbon dioxide, carbon monoxide, ammonia,
hydrogen chloride, oxides of nitrogen.
Hazardous Polymerization: Will not occur
Conditions To Avoid: Any conditions which are incompatible
with water, incompatible chemicals.
11. TOXICOLOGICAL INFORMATION
Toxicity Data: The specific toxicology data available for components greater that 1% in concentration are as follows:
FORMAMIDE:
LD50 (intra peritoneal, rat) = 5700 mg/kg
LD50 (oral, rat) = 5570 mg/kg
LD50 (intra peritoneal, mouse) = 2450 mg/kg
LD50 (oral, mouse) = 3150 mg/kg
GLYCERINE:
LD50 (intra peritoneal, rat) = 4420 mg/kg
LD50 (subcutaneous, rat) = 1000 mg/kg
LD50 (oral, mouse) = 4090 mg/kg
LD50 (intra peritoneal, mouse) = 8982 mg/kg
LD50 (subcutaneous, mouse) = 91mg/kg
LD50 (intravenous, mouse) = 4250 mg/kg
TRIS(HYDROXYMETHYL)AMINOMETHANE HYDROCHLORIDE (TRIS-HCl):
Currently, there are
no toxicological data available for this compound.
Suspected Cancer Agent: The chemicals in the product's solutions
are not found on the following lists: NTP, IARC, Federal OSHA Z-list and CAL-OSHA,
and therefore are not considered to be, nor suspected by these agencies, to
be cancer causing agents.
Irritancy Of Product: BLUE STOP SOLUTION: Prolonged skin over-exposure
to this component may cause dermatitis (dry, red skin). Eye over-exposure
may cause temporary cornea damage.
ALL OTHER SOLUTIONS:
Although not tested, the other components of this product are not expected to
cause irritancy to the skin. Mild eye irritation may occur if the other
components of this product enter the eye.
Sensitization To The Product: The chemicals in this product's
solutions are not know to be sensitizer with prolonged or repeated use.
Reproductive Toxicity Information: Listed below is information
concerning the effects of this product and its components on the human reproductive
system.
Mutagenicity: This product is not reported to produce mutagenic
effects in humans. Mutation data are available for Formamide (a chemical
in this product's solutions); these data were obtained during clinical studies
on specific animal tissues exposed to high doses of this compound.
Embryo toxicity: This product is not reported to cause human
teratogenic effects. Refer to the following paragraph for additional information.
Teratogenicity: This product is not reported to cause human
teratogenic effects. Clinical studies on test animals exposed to relatively
high doses of Formamide indicate teratogenic effects.
Reproductive Toxicity: This product is not reported at this
time to cause adverse reproductive effects in humans. Clinical studies
on test animals exposed to relatively high doses of Formamide indicate teratogenic
effects.
Medical Condition Aggravated By Exposure: Skin disorders may
be aggravated by over-exposure to the BLUE STOP SOLUTION. No specific
medical conditions are known to be aggravated by exposure to other solutions
of this product.
Recommendation To Physicians: This product is not expected
to cause clinical symptoms. If symptoms occur, treat the symptoms and
eliminate over-exposure.
Biological Exposure Indices (BEI's): Currently, there are no
Biological Exposure Indices (BEI's) applicable to the components of this product.
12. ECOLOGICAL INFORMATION
Environmental Stability: The chemicals in
this product's solutions will degrade in the environment into organic and inorganic
constituents.
Effect Of Material On Plants Or Animals: No unusual effects
on plants or animals are expected if this product is released into the environment.
See Section 11 (Toxicological Information) for further information regarding
the effects of chemicals in this product's solutions on test animals.
13. DISPOSAL CONSIDERATIONS
Preparing Wastes For Disposal: Waste Disposal
must be in accordance with appropriate Federal, State and local regulation.
This product, if altered by use, may be disposed of by treatment at a permitted
facility or as advised by your local hazardous waste regulatory authority.
EPA Waste Number: Not applicable to this product.
14. TRANSPORTATION INFORMATION
This material is not hazardous as defined by 49 CFR 172.101 by the U.S. Department of Transportation. This material is not considered as Dangerous Goods.
Proper Shipping
Name: N/A
Hazard Class Number And Description: N/A
UN Identification Number: N/A
Packing Group: N/A
15. REGULATORY INFORMATION
SARA Reporting Requirements: The following chemical is subject to Sections 302, 304, and 313 reporting requirements under the Superfund Amendment and Reauthorization Act.
Chemical SARA 302 SARA 304 SARA 313
Ethylenediaminetetraacetic acid NO YES NO
(EDTA)
SARA Threshold Planning Quantity: N/A
TSCA Inventory Status: The components of this product are listed
on the TSCA inventory.
CERCLA Reportable Quantity
(RQ): EDTA = 5000 lbs.
Other Federal Regulations: N/A
FOR BLUE STOP SOLUTION
Labeling (precautionary statements): WARNING! MAY CAUSE EYE AND SKIN IRRITATION
16. OTHER INFORMATION
|
Created: 03/11/02 |
Prepared By LI-COR, Inc.
|